Journal: International Journal of Molecular Sciences

Article Title: Extracellular Matrix Expression in Human Pancreatic Fat Cells of Patients with Normal Glucose Regulation, Prediabetes and Type 2 Diabetes

doi: 10.3390/ijms241311169

Figure Lengend Snippet: ( A – N ) Relative mRNA levels in PPAs after treatment with 600 μg/mL fetuin-A + 60 µmol/L palmitate for 24h (black bars) versus untreated controls (white bars). Data from all of the individuals of the 3 groups are pooled. ΔCT values are shown (related to the housekeeping gene RPS13 . Data are expressed as the mean ± SEM ( n = 5–7, Student’s t -test, * p < 0.05, ** p < 0.01).

Article Snippet: Half of the mono- and co-cultures were treated with fetuin-A (alpha-2-HS-glycoprotein, Sigma, München, Germany, endotoxin <0.05 EU/μg [0.005 ng/μg] determined by ELISA kit Hycult Biotech, Uden, the Netherlands).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Extracellular Matrix Expression in Human Pancreatic Fat Cells of Patients with Normal Glucose Regulation, Prediabetes and Type 2 Diabetes

doi: 10.3390/ijms241311169

Figure Lengend Snippet: ( A – I ) Protein levels in PPAs after treatment with 600 μg/mL fetuin-A + 60 μmol/L palmitate for 24 h (dark grey bars) versus untreated controls (white bars). The data from all individuals in the 3 groups are pooled. Protein concentrations were (pg/mL) determined using the Luminex technology (see methods). Data are expressed as the mean ± SEM ( n = 5–7, Student’s t -test, * p < 0.05).

Article Snippet: Half of the mono- and co-cultures were treated with fetuin-A (alpha-2-HS-glycoprotein, Sigma, München, Germany, endotoxin <0.05 EU/μg [0.005 ng/μg] determined by ELISA kit Hycult Biotech, Uden, the Netherlands).

Techniques: Luminex

Journal: International Journal of Molecular Sciences

Article Title: Extracellular Matrix Expression in Human Pancreatic Fat Cells of Patients with Normal Glucose Regulation, Prediabetes and Type 2 Diabetes

doi: 10.3390/ijms241311169

Figure Lengend Snippet: Relative mRNA levels of human islets in mono-cultures and in co-cultures with PPAs, as well as under the influence of 600 μg/mL fetuin-A/60 μmol/L palmitate for 24 h. ( A ) The effect of co-cultured PPAs on islet mRNA levels (fold-change, grey bars) compared with respective mRNA levels in mono-cultured islets (black bar, defined as 1, under the same conditions). ( B ) The effect of co-cultured PPAs and fetuin-A/palmitate treatment on islet mRNA levels (fold-change) compared with untreated controls (black bars, defined as 1) under the same conditions. Data are expressed as the mean ± SEM ( n = 3, Student’s t -test, * p < 0.05, ** p < 0.01).

Article Snippet: Half of the mono- and co-cultures were treated with fetuin-A (alpha-2-HS-glycoprotein, Sigma, München, Germany, endotoxin <0.05 EU/μg [0.005 ng/μg] determined by ELISA kit Hycult Biotech, Uden, the Netherlands).

Techniques: Cell Culture

Journal: International Journal of Molecular Sciences

Article Title: Extracellular Matrix Expression in Human Pancreatic Fat Cells of Patients with Normal Glucose Regulation, Prediabetes and Type 2 Diabetes

doi: 10.3390/ijms241311169

Figure Lengend Snippet: ( A ) Migration assay: human monocytes seeded on the upper filter side of a Transwell system were co-cultured with human PPAs-NGT or PPAs-T2D on the bottom of the plate. Then, 600 µg/mL fetuin-A ± 60 µmol/L palmitate were added to the culture medium of the lower compartment. MCP-1 was used as a ‘positive’ control (grey bars). ( B ) Results for PPAs-NGR. ( C ) Results for PPAs-T2D. All results are compared to untreated PPAs without monocytes (controls) cultured with standard culture medium (white bars). The third column shows the co-culture of PPAs with monocytes in standard culture medium and the fourth column after treatment with fetuin-A/palmitate. Data are expressed as the mean ± SEM ( n = 3, Student’s t -test, * p < 0.05, ** p < 0.01).

Article Snippet: Half of the mono- and co-cultures were treated with fetuin-A (alpha-2-HS-glycoprotein, Sigma, München, Germany, endotoxin <0.05 EU/μg [0.005 ng/μg] determined by ELISA kit Hycult Biotech, Uden, the Netherlands).

Techniques: Migration, Cell Culture, Positive Control, Co-Culture Assay

Journal: PLoS ONE

Article Title: Proteomic Analyses Reveal High Expression of Decorin and Endoplasmin (HSP90B1) Are Associated with Breast Cancer Metastasis and Decreased Survival

doi: 10.1371/journal.pone.0030992

Figure Lengend Snippet: Antibodies used to validate putative biomarkers.

Article Snippet: Alpha-2-HS-glycoprotein precursor (AHSG) , Sigma-Aldrich.

Techniques:

Journal: Journal of Clinical Medicine

Article Title: Proteomic Analysis of Aqueous Humor Proteins in Association with Cataract Risks: Diabetes and Smoking

doi: 10.3390/jcm10245731

Figure Lengend Snippet: List of aqueous humor (AH) proteins identified by LC-ESI-MS/MS.

Article Snippet: An alpha-2-Heremans–Schmid (HS)-glycoprotein ELISA assay was performed to measure concentrations of AH samples among the single-risk group, double-risk group, and the age-matched cataract controls with a Human Alpha-2-HS-glycoprotein ELISA Kit (EH310RB, ThermoFisher Scientific), as per the manufacturer’s protocol.

Techniques: Ubiquitin Proteomics

Journal: Journal of Clinical Medicine

Article Title: Proteomic Analysis of Aqueous Humor Proteins in Association with Cataract Risks: Diabetes and Smoking

doi: 10.3390/jcm10245731

Figure Lengend Snippet: List of selected potential biomarker candidates.

Article Snippet: An alpha-2-Heremans–Schmid (HS)-glycoprotein ELISA assay was performed to measure concentrations of AH samples among the single-risk group, double-risk group, and the age-matched cataract controls with a Human Alpha-2-HS-glycoprotein ELISA Kit (EH310RB, ThermoFisher Scientific), as per the manufacturer’s protocol.

Techniques: Biomarker Discovery, Control, Ubiquitin Proteomics

Journal: Frontiers in Cell and Developmental Biology

Article Title: Proteomic Changes Associated With Sperm Fertilizing Ability in Meat-Type Roosters

doi: 10.3389/fcell.2021.655866

Figure Lengend Snippet: Differentially abundant proteins between fertile (F) and subfertile (SF) meat-line chickens identified by GeLC-MS/MS.

Article Snippet: 293 , Alpha-2-HS-glycoprotein [ Gallus gallus ] , XP_422764.1 , 37 , AHSG , 0.028 , 0.00 , 0.014 , 0.00.

Techniques:

Journal: Malaria Journal

Article Title: The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity

doi: 10.1186/s12936-020-03229-1

Figure Lengend Snippet: Serum proteins were selectively taken up by Plasmodium falciparum . a Western blotting analysis of serum proteins. “Serum” and “Lysate” indicate plasma-derived serum (PDS) and isolated parasite lysate prepared from infected red blood cells (iRBCs) cultured in PDS-containing medium. Samples were prepared using the Percoll method. SERA5 was used as an internal control. Protein S vitamin K-dependent protein S, AHSG alpha-2-HS-glycoprotein, C4BPA C4b-binding protein alpha chain, SERA5 serine repeat antigen 5. The asterisk indicates a non-specific band. b Confocal microscopy images showing localization of prothrombin (green), EXP2/3C (red), and DNA (cyan). Samples were fixed using the paraformaldehyde/glutaraldehyde fixation method. Scale bar represents 5 µm. c Confocal microscopy images showing the localization of prothrombin (red) and DNA (cyan). Samples were fixed using the methanol fixation method. The scale bar represents 5 µm. All samples were prepared from 3D7 strain

Article Snippet: Anti-protein S polyclonal antibody (pAb) (16910-1-AP), anti-prothrombin pAb (24295-1-AP), anti-vitronectin pAb (15833-1-AP), anti-alpha-2-HS-glycoprotein pAb (16571-1-AP), anti-alpha-2-macroglobulin pAb (13545-1-AP), anti-band 3 pAb (18566-1-AP), anti-fibrinogen beta chain pAb (16747-1-AP), and rabbit control IgG (30000-0-AP) were obtained from Proteintech (Rosemont, IL, USA).

Techniques: Western Blot, Clinical Proteomics, Derivative Assay, Isolation, Infection, Cell Culture, Control, Binding Assay, Confocal Microscopy

Journal: Malaria Journal

Article Title: The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity

doi: 10.1186/s12936-020-03229-1

Figure Lengend Snippet: List of serum proteins internalized in malaria-infected red blood cells (iRBCs)

Article Snippet: Anti-protein S polyclonal antibody (pAb) (16910-1-AP), anti-prothrombin pAb (24295-1-AP), anti-vitronectin pAb (15833-1-AP), anti-alpha-2-HS-glycoprotein pAb (16571-1-AP), anti-alpha-2-macroglobulin pAb (13545-1-AP), anti-band 3 pAb (18566-1-AP), anti-fibrinogen beta chain pAb (16747-1-AP), and rabbit control IgG (30000-0-AP) were obtained from Proteintech (Rosemont, IL, USA).

Techniques: Clinical Proteomics